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rabbit anti psd 95  (Thermo Fisher)


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    Structured Review

    Thermo Fisher rabbit anti psd 95
    Rabbit Anti Psd 95, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+psd+95/Nunc+Seals/bio_rxiv__2025__10__14__682478-64-28-34
    Average 99 stars, based on 1 article reviews
    rabbit anti psd 95 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Distal retinal ganglion cell axon transport loss and activation of p38 MAPK stress pathway following VEGF-A antagonism
    Article Snippet: .. Sections were blocked in 20% NGS for 1 h at room temperature (RT), before rabbit anti-PSD-95 (1:200; Life Technologies) and mouse anti-synaptophysin (1:500; Abcam, Cambridge, UK) antibodies in 0.3% Triton X-100 in PBS (T-PBS) with 10% NGS were added overnight. ..

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: 40 μm slices were rinsed three times in tris-buffered saline (TBS; pH 7.6) in 0.2% triton 100-X (TBS + 0.2% triton 100-X; TBST) and blocked at room temperature for 1 hour in 10% normal goat serum (NGS; cat# 005-000-121; Jackson Immunolabs, West Grove, PA) and TBST. .. Slices were incubated overnight in mouse anti-ezrin (1:500; cat# SAB4200806; RRID:AB_3714841; Sigma-Aldrich, St. Louis, MO), guinea pig anti-bassoon (1:500; cat# 141-318; RRID: AB_2927388; Synaptic Systems, Göttingen, Germany), and rabbit anti-PSD-95 (1:350; cat# 51-6900; RRID:AB_2533914; Thermo Fisher Scientific, Waltham, MA) in 10% NGS and TBST at 4 °C. .. Slices were washed 3 times in TBST then incubated for 2 hours at room temperature in goat anti-guinea pig 488 (1:100; Alexa Fluor cat# A11073; RRID:AB_2534117; Thermo Fisher Scientific, Waltham, MA), goat anti-mouse 594 (1:100; Alexa Fluor cat# A21125; RRID:AB_2535767; Thermo Fisher Scientific, Waltham, MA), and goat anti-rabbit Atto 647N (1:100; cat# 611-156-122; RRID:AB_10893043; Rockland Immunochemicals, Limerick, Pennsylvania) in 10% NGS and TBST.

    Incubation:

    Article Title: Adolescent Alcohol Exposure Disrupts Astrocyte-Synaptic Structural And Functional Coupling In The Male Dorsal Hippocampus
    Article Snippet: 40 μm slices were rinsed three times in tris-buffered saline (TBS; pH 7.6) in 0.2% triton 100-X (TBS + 0.2% triton 100-X; TBST) and blocked at room temperature for 1 hour in 10% normal goat serum (NGS; cat# 005-000-121; Jackson Immunolabs, West Grove, PA) and TBST. .. Slices were incubated overnight in mouse anti-ezrin (1:500; cat# SAB4200806; RRID:AB_3714841; Sigma-Aldrich, St. Louis, MO), guinea pig anti-bassoon (1:500; cat# 141-318; RRID: AB_2927388; Synaptic Systems, Göttingen, Germany), and rabbit anti-PSD-95 (1:350; cat# 51-6900; RRID:AB_2533914; Thermo Fisher Scientific, Waltham, MA) in 10% NGS and TBST at 4 °C. .. Slices were washed 3 times in TBST then incubated for 2 hours at room temperature in goat anti-guinea pig 488 (1:100; Alexa Fluor cat# A11073; RRID:AB_2534117; Thermo Fisher Scientific, Waltham, MA), goat anti-mouse 594 (1:100; Alexa Fluor cat# A21125; RRID:AB_2535767; Thermo Fisher Scientific, Waltham, MA), and goat anti-rabbit Atto 647N (1:100; cat# 611-156-122; RRID:AB_10893043; Rockland Immunochemicals, Limerick, Pennsylvania) in 10% NGS and TBST.

    Article Title: Modulation of Both Intrinsic and Extrinsic Factors Additively Promotes Rewiring of Corticospinal Circuits after Spinal Cord Injury
    Article Snippet: Serial 20- or 50-mm-thick sections were made with a cryostat and mounted on SuperFrost Plus slides (Fisher Scientific) or MAS-coated slides (Matsunami). .. For immunohistochemistry, the sections were blocked with 1% bovine serum albumin (BSA) in 0.3% Triton X-100/PBS for 2 h and then incubated with the following primary antibodies overnight at 4°C: mouse anti-GFAP (1:400; catalog #G3898, Sigma-Aldrich), rabbit anti-GFP (1:1000; catalog #A11122, Invitrogen), sheep anti-GFP (1:1000; catalog #4745-1051, Serotec), rabbit anti-Pten (1:100; catalog #9188, Cell Signaling Technology), rabbit anti-phospho-S6 ribosomal protein (1:400; catalog #4858, Cell Signaling Technology), rabbit anti-RFP antibody (1:1000; catalog #600- 401-379, Rockland), goat anti-mCherry (1:1000; catalog #AB0040-200, Sicgen), guinea pig anti-Vglut1 (1:10,000; catalog #AB5905, Millipore), or rabbit anti-PSD-95 (1:200; catalog #516900, Invitrogen). .. After washing with 0.1% Tween 20/PBS, the sections were incubated with the following secondary antibodies for 2 h at room temperature: Alexa Fluor 488 or 568 donkey anti-rabbit, mouse, sheep, or goat IgG antibody (1:500; Invitrogen) or Alexa Fluor 647 donkey anti-guinea pig IgG antibody (1:1000; Jackson ImmunoResearch).

    Article Title: The Cortical Motor System in the Domestic Pig: Origin and Termination of the Corticospinal Tract and Cortico-Brainstem Projections
    Article Snippet: As a first step in identifying spinal synaptic contacts by CST axons, immunohistochemistry for the postsynaptic density protein (PSD-95) was additionally performed in transverse sections of the C3 segment of pig 3. .. 16-μm-thick sections were unmasked for antigen retrieval by heating at 90°C for 20 min with 0.01 M sodium citrate; after, they were blocked for 1 h in PBS containing 0.2% triton and 2% normal donkey serum, rinsed three times with PBS, and incubated overnight at 4°C with rabbit anti-PSD-95 (Invitrogen 51-6900; 1:100), diluted in phosphate buffer with 0.2% triton and 1% normal donkey serum. .. The sections were then washed with phosphate buffer and incubated for 2 h at room temperature with donkey anti-rabbit secondary fluorescent antibody (DyLight 405; Jackson ImmunoResearch 711-475-152; 1:100).

    Immunohistochemistry:

    Article Title: Modulation of Both Intrinsic and Extrinsic Factors Additively Promotes Rewiring of Corticospinal Circuits after Spinal Cord Injury
    Article Snippet: Serial 20- or 50-mm-thick sections were made with a cryostat and mounted on SuperFrost Plus slides (Fisher Scientific) or MAS-coated slides (Matsunami). .. For immunohistochemistry, the sections were blocked with 1% bovine serum albumin (BSA) in 0.3% Triton X-100/PBS for 2 h and then incubated with the following primary antibodies overnight at 4°C: mouse anti-GFAP (1:400; catalog #G3898, Sigma-Aldrich), rabbit anti-GFP (1:1000; catalog #A11122, Invitrogen), sheep anti-GFP (1:1000; catalog #4745-1051, Serotec), rabbit anti-Pten (1:100; catalog #9188, Cell Signaling Technology), rabbit anti-phospho-S6 ribosomal protein (1:400; catalog #4858, Cell Signaling Technology), rabbit anti-RFP antibody (1:1000; catalog #600- 401-379, Rockland), goat anti-mCherry (1:1000; catalog #AB0040-200, Sicgen), guinea pig anti-Vglut1 (1:10,000; catalog #AB5905, Millipore), or rabbit anti-PSD-95 (1:200; catalog #516900, Invitrogen). .. After washing with 0.1% Tween 20/PBS, the sections were incubated with the following secondary antibodies for 2 h at room temperature: Alexa Fluor 488 or 568 donkey anti-rabbit, mouse, sheep, or goat IgG antibody (1:500; Invitrogen) or Alexa Fluor 647 donkey anti-guinea pig IgG antibody (1:1000; Jackson ImmunoResearch).



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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.
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    Image Search Results


    Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Journal: Frontiers in Endocrinology

    Article Title: Dorzagliatin shows potential in preventing cognitive impairment in diabetes: evidence from Mendelian randomization analysis and animal study

    doi: 10.3389/fendo.2025.1755359

    Figure Lengend Snippet: Dorzagliatin prevented diabetes-induced downregulation of synaptic proteins in Goto Kakizaki rats. (A) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in hippocampus of each group. GAPDH was used as an internal control. (B–D) Statistics of GluN1 (B) , GluN2A (C) and PSD-95 (D) protein levels in the hippocampus of T2D (Goto Kakizaki-vehicle) and control (Wistar-vehicle). (E) Western blot analysis of selected glutamate receptors and postsynaptic density protein 95 (PSD-95) in the hippocampus of Goto Kakizaki-vehicle and Goto Kakizaki-dorzagliatin rats. (F–H) Statistics of GluN1 (F) , GluN2A (G) and PSD-95 (H) protein levels in the hippocampus of Goto Kakizaki-vehicle group and Goto Kakizaki-dorzagliatin group. Data are expressed as mean ± SEM. Student’s t test, two tailed. *P < 0.05, **P < 0.01. (n=4 per group). hippo, hippocampus; dorza, dorzagliatin.

    Article Snippet: The membranes were blocked with 1×TBST and 5% BSA (tank blotting) or 5% skim milk (Semi-dry blotting) for 1 hour at room temperature and then incubated overnight at 4°C with following primary antibodies, respectively: mouse monoclonal anti-GluN1 antibody (Millipore, Cat. No: 05-432); rabbit polyclonal anti-GluN2A antibody (NOVUS, Cat. No: NB300-105); rabbit monoclonal anti-GLUT1 antibody (Abcam, Cat. No: ab115730); rabbit polyclonal anti-GLUT3 antibody (Bioss, Cat. No: bs-1207R); rabbit polyclonal anti-IR antibody (Abcam, Cat. No: ab137747); rabbit monoclonal anti-PSD95 antibody (Abcam, Cat. No: ab238135).

    Techniques: Western Blot, Control, Two Tailed Test